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. 2019 Feb 14;13:39. doi: 10.3389/fncel.2019.00039

Figure 5.

Figure 5

Effect of frame rate on SNR and spike timing estimation. Decreasing the imaging rate in wide-field imaging reduces the temporal accuracy and AP shape information but increases the SNR. (A) Image of a brain slice from sparse GEVI mouse obtained at 500 Hz frame rate (80 × 80 pixels). Simultaneous electrical recording from cell body (black) and optical recording (red). Average of nine trials. (B) A 500 Hz ΔF/F trace with 3 single-spike transients and down samples by averaging. Signal size, noise level and AP timing estimation accuracy all decrease with mean downsampling. (C,D) Power law fits to 1—signal size and the noise level. The noise level scales approximately with the square as expected from Poisson statistics. (E) The signal to noise ratio and power law fits plotted on linear axes. Fits give an optimal frame rate for spike detection SNR of 99 Hz. Note that this curve is the result of the division of the log-log linear fits in (C,D) and not a fit to the plotted points. (F) The bounds on spike timing estimation for different frame rates. Fluorescence traces shown on inverted y axes.