Table 2.
Exoerythrocytic form development is supported following sporozoite invasion.
| Rep 1 MSP-1 % (N) | Rep 2 MSP-1 % (N) | Rep 3 MSP-1 % (N) | Rep 4 MSP-1 % (N) | Rep 5 MSP-1 % (N) | Rep 6 MSP-1 % (N) | HSP70 % (N) | |
|---|---|---|---|---|---|---|---|
| HC-04.J7 | 4.2 (20) | 4.2 (20) | 4.9 (12) | 4.7 (12) | 3.7 (12) | 7.4 (17) | 6.9 (20) |
| HC-04 | 1.6 (20) | 2.7 (20) | 1.5 (12) | 1.4 (12) | 1.0 (12) | 3.6 (17) | 1.5 (19) |
%, percent infection, N = # of fields read. Rep, Biological replicate number. Merozoite surface protein-1 (MSP-1) staining with mAb 5.2-Alexa488 conjugated. P. falciparum HSP70 staining with pAb. Coverslips with HC-04.J7 or HC-04 cells were harvested and fixed 5 days post-infection and stained with anti-MSP-1 or HSP70 antibodies; the percentage of HC-04 cells containing developing exoerythrocytic forms is reported.