Table 3.
Proteins enriched in HC-04.J7 compared to HepG2.
| Identified proteins | Accession number | Molecular mass | Extracellular membrane protein? | Cell receptor? | Fold expression HC-04.J7/HepG2 | P-value |
|---|---|---|---|---|---|---|
| Integrin alpha-2 | ITA2_HUMAN | 129 kDa | YES | YES | 6.29 | 0.00291 |
| Glypican-3 | GPC3_HUMAN | 66 kDa | YES | YES | 69.61 | 0.00298 |
| B-cell receptor-associated protein 29 | BAP29_HUMAN | 28 kDa | YES | YES | 30.16 | 0.00474 |
| Transferrin receptor protein 1 | TFR1_HUMAN | 85 kDa | YES | YES | 3.14 | 0.02576 |
| Integrin alpha-V | ITAV_HUMAN | 116 kDa | YES | YES | 47.89 | 0.02995 |
| Transient receptor potential cation channel subfamily V member 2 | TRPV2_HUMAN | 86 kDa | YES | YES | 18.70 | 0.03006 |
Proteins with a fold change greater than 1.5 and a P-value < 0.05 when HC-04.J7 and HepG2 normalized spectral counts are compared that are also extracellular membrane proteins with reported receptor function. Glypican-3 was selected for further analysis and is presented in bold here. P-values were calculated using a two-tailed Student’s t-test comparing two samples with equal variance.