Figure 5.

Cold-induced reduction of miR173 and TAS1a-derived siRNAs. Small RNA gel blot analysis was performed using RNA from rosette leaves harvested at different time-points after application of cold. Samples were hybridized with radioactively labeled probes specific to miR173 and the different siRNAs and bands were quantified. (A) miR173, (B) siR752, (C) siR255, and (D) siR477. The temperature at the different time-points was 20°C (0 min), 5°C (90 min), and 4°C (120 and 180 min). The letters on the bars indicate significantly different expression levels of small RNAs using Student’s t-test (p < 0.05); different letters mean significant differences between the samples.