Fig. 5.

Effects of PNA5 treatment on NOR task performance in HF and control mice. (A) Mean discrimination ratios, taken from the first 2 minutes of test phase, of HF and control mice after 3 weeks of treatment with PNA5 and 3 days after the last injection. A positive score indicates more time spent with the novel object, whereas a negative score indicates more time spent with the familiar object. A zero score indicates a null preference. PNA5 rescued object recognition performance. HF-PNA5 mice (n = 11) NOR discrimination ratios were similar to those of controls (n = 6) and significantly greater than those of the HF-saline mice (n = 10) (+0.38 ± 0.1 vs. −0.05 ± 0.09; F(3,28) = 8.13, P = 0.0004, ANOVA, *P < 0.05). (B) During the familiarization phase with two identical objects, there was a null preference for the two identical objects for HF+PNA5 mice, the HF+saline mice and the control mice (−0.04 ± 0.05, −0.06 ± 0.1, and −0.007 ± 0.1, respectively). These results suggest that there was no difference in object preference for identical objects during the familiarization phase.