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. 2019 Jan 24;4(2):e123862. doi: 10.1172/jci.insight.123862

Figure 3. Flow cytometry profiling of myeloid cells at day 7 and day 21 after mesh implantation in mice.

Figure 3

Flow cytometric characterization of mesh explants: t-SNE flow cytometry analysis of murine mesh explants at day 7 (A) and day 21 (B) after implantation. Cells were pregated on CD45+Ly6G– cells and subjected to unbiased t-SNE analysis. Overlays were generated from mesh- and sham-operated animals, and the clusters were then analyzed in detail for their marker expression profiles, as shown in the subsequent histogram overlays. MAM, mesh-associated macrophage; SIM, stroma-infiltrating macrophage. (C) Cytokine production from myeloid cells isolated from mesh-associated tissue. Cells were pregated on CD45+Ly6G–F480+CD11b+. (D) Phenotyping of TNF+ cells pregated on CD45+Ly6G–. Exemplary results are shown in the dot plots; statistical analysis was performed from ≥5 animals per group. Student’s t test: *P < 0.05; ***P < 0.001. Error bars represent mean ± SD.