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. 2019 Feb 7;4(3):e123384. doi: 10.1172/jci.insight.123384

Figure 1. Anergic BND cells (representing 2.5% of B cells) are hyporesponsive and have elevated expression of PTEN.

Figure 1

(A) Gating strategy for BND cells and mature naive (MN) B cells in healthy subjects. (B) BND cells have an elevated basal calcium and decreased calcium flux following stimulation with 10 μg/ml F(ab′)2 rabbit anti-human IgG H&L. (C) BND cells have decreased pSyk, pPLCy2, pAkt, and pBtk compared with MN cells following stimulation used as in B. (D) Representative histogram showing PTEN expression is increased in BND cells compared with MN cells and isotype control. (E) PTEN expression is increased in BND cells compared with MN cells. Phosflow samples were run and analyzed in triplicates. Data depict results from triplicate samples from the same individual; data are representative of n = 12 independent experiments. Statistics were determined using Mann-Whitney unpaired t test; **P < 0.01, ***P < 0.001.