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. 2019 Feb 7;4(3):e123384. doi: 10.1172/jci.insight.123384

Figure 2. PTEN limits signaling in anergic B cells.

Figure 2

(A) miRNA-7, -21, and -92a (but not miRNA-22) are decreased in BND cells compared with mature naive (MN) B cells; the miRNA comparison was compiled from n = 5 healthy individuals. Statistics were determined using unpaired Mann-Whitney t test. (B) Inhibition of PTEN with 325 μM SF1670 restores Ca2+ mobilization in BND cells to similar levels to MN B cells. Representative plot shown for n = 5 healthy individuals. (C) The change in the AUC in BND and MN B cells following addition of SF1670, ran in triplicates. Data are representative of n = 5 healthy individuals. (D and F) Inhibition of PTEN with SF1670 causes an elevation in basal pPLCy2 and pAkt in BND cells, (E and G) without an overall increase in change in pPLCy2 or pAkt following stimulation. (H and I) Basal and changes in pSyk following stimulation were unchanged following addition of the PTEN inhibitor in BND cells. Phosflow samples were run and analyzed in triplicates. Data depict results from triplicate samples from the same individual; data is representative of n = 9 independent experiments. Statistics were determined using 1-way ANOVA followed by a Bonferroni’s multiple comparison post-test test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.