Figure 4. Tollip^–/– neutrophils facilitated T cell activation and survival.

(A) Splenocytes were cocultured with GM-CSF–primed neutrophils (WT or Tollip^–/–) in anti-CD3 antibody–coated plates for 24 hours, and then CD62L levels on CD4⁺ or CD8⁺ T cells were measured by flow cytometry. Representative results are shown. (B) After coculture, CD69 levels on CD4⁺ cells and CD107a⁺ cells in CD8⁺ cells were analyzed. (C) Conditional medium from coculture was analyzed by ELISA. (D) Splenocytes were cocultured with GM-CSF–primed neutrophils (WT or Tollip^–/–) for 72 hours, before cell viabilities were tested. (E) Quantification analysis of the cell viabilities. Statistical significance compared with WT in the same treatment conditions was determined by Mann-Whitney U test (B and C) or Student’s t test (E). *P < 0.05, **P < 0.01, ***P < 0.01.