Figure 6. Adoptive transfer of Tollip^–/– neutrophils to WT mice slows down colitis-associated cancer progression.

(A) Representative images of colons on day 64 from WT mice that received WT or Tollip^–/– neutrophils. (B) Graphical representation of tumor burden in WT mice that received WT or Tollip^–/– neutrophils. n ≥ 5 per group. (C) H&E-stained sections of colons from the mice that received WT or Tollip^–/– neutrophils. Colons were collected in Swiss rolls at the end of the AOM-DSS regimen. Scale bars: 2.5 mm (top) and 0.5 mm (bottom). (D) Immunofluorescence analysis of Ki67 and β-catenin. Blue color is DAPI staining. Scale bars: 200 μm. (E) CD4⁺ and CD8⁺ cell counts in the spleens from the mice that received WT or Tollip^–/– neutrophils. (F) Percentages of CD62L^lo in CD8⁺ T cells. Percentage of granzyme B–positive (GrzB⁺) cells in CD8⁺ T cells. Statistical significance compared with WT in the same treatment conditions was determined by Student’s t test (B) or Mann-Whitney U test (E and F). *P < 0.05, **P < 0.01, ***P < 0.001.