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Establishment of double-gene expression in Huh7 cells.
Notes: (A) CMV promoter driven hNIS and EGFP was expressed in cells. (B) Western blotting analysis for the finding of NIS proteins from Huh7/NIS cells, not from Huh7 cells. (C) Confocal microscopy analysis to detect NIS and GFP protein in Huh7/NIS cells, not from Huh7 cells.
Abbreviations: CMV, cytomegalovirus; IRES, internal ribosomal entry site.
