Skip to main content
. 2019 Jan 24;4(2):e125067. doi: 10.1172/jci.insight.125067

Figure 5. Diminished GP-specific CD8+ effector T cell responses in Rip-LysM-Ifnarfl/fl mice.

Figure 5

(A) Frequencies of LCMV-GP33–41 tetramer–positive CD8+ T cells in the spleens and PLNs at day 11 p.i. (B) Median fluorescence intensity (MFI) of PD-1 and KLRG1 at the surface of LCMV-GP33–41 tetramer–positive CD8+ T cells at day 11 p.i. (C) Detection of IFN-γ, TNF-α, and IL-2 in CD8+ T cells following ex vivo restimulation of splencoytes from Rip-LysM-Ifnarfl/fl mice with LCMV-GP33–41 peptide at day 11 p.i. (D) Specific lysis of target splenocytes loaded with CellTrace violet (CTV) and LCMV-GP33–41 (H-2b, CTVhi population) or NP118–126 (H-2d, CTVlo population) peptides. Statistical significance was measured using an unpaired 2-tailed Student t test, one-way ANOVA analysis or 2-way ANOVA analysis. Data presented as average ± SEM. ***P < 0.001, ****P < 0.0001.