Figure 1. GL-2045 is composed of ordered Fc multimers and effectively binds immune cells from different species.

(A) Schematic illustration of the structure of the GL-2045 homodimer composed of the human IgG1 CH2 and CH3 domains and the IgG2 hinge region. (B) SDS-PAGE analysis of GL-2045 under nonreduced and reduced conditions. (C) FACS analysis of GL-2045 and G001 binding to immune cells of human, nonhuman primate (cynomolgus macaque), and mouse origin. For human peripheral blood analysis, T cells/B cells/NK cells were identified as CD3⁺/CD3^–CD19⁺/CD3^–CD56⁺ within the lymphocyte gate, monocytes were characterized as CD14⁺ cells within the monocyte gate, and granulocytes were identified based on FSC vs. SSC. For cynomolgus macaque peripheral blood, T cells/B cells/NK cells were identified as CD3⁺/CD3^–CD19⁺/CD3^–CD16⁺ within the lymphocyte gate, monocytes were characterized as CD14⁺ cells within the monocyte gate, and granulocytes were identified based on FSC vs. SSC. In murine spleen, the markers included to identify different cell populations were: CD3⁺ (T cells), CD3^–Dx5⁺ (NK cells), CD3^–B220⁺ (B cells), B220^–/CD11b⁺/Gr1^–/int (mono/macrophages), and B220^–/CD11b⁺/Gr1^hi (granulocyte). Data represent 1 of at least 3 different experiments using cells from different donors.