Table 2.
Replication of rMV-Hu191 mutants in cotton rats.
| Viral replication in lunga |
||
|---|---|---|
| Inoculum | % infected animals | Viral titer (log10 PFU/g)b,c |
| rMV-Hu191 | 100 | 3.75 ± 0.12A |
| rMV-Hu191-G1788A | 100 | 3.08 ± 0.33B |
| rMV-Hu191-G1792A | 20 | 2.56B |
| DMEM | 0 | ND |
ND: not detected.
a
Each cotton rat was inoculated intranasally with 5.0 × 106 PFU of rMV-Hu191 or rMV-Hu191 mutants in a volume of 100 µl. At day 4 post-infection, the cotton rats were sacrificed, and lungs were collected for both virus titration and RT-PCR.
b
The viral titer was determined by plaque assay.
c
Five cotton rats were tested in each group. Values within a column followed by different capital letters (A and B) are significantly different.