Table 3.
Immunogenicity of MTase-defective rMV-Hu191 mutants in cotton ratsa.
| Viral replication in lung |
||
|---|---|---|
| Inoculuma | % infected animals | Viral titer (log10 PFU/g)b |
| rMV-Hu191 | 0 | ND |
| rMV-Hu191-G1788A | 0 | ND |
| rMV-Hu191-G1792A | 0 | ND |
| DMEM | 100 | 4.02± 0.37 |
ND: Not detected.
a
Cotton rats were intranasally inoculated with 1.0 × 106 PFU of rMV-Hu191 or mutants. At 28 days post-infection, rats were challenged with 1 × 107 PFU of rMV-Hu191. At day 4 post-challenge, cotton rats were sacrificed and lungs were collected for both virus titration and RT-PCR.
b
The viral titer was determined by plaque assay.