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. 2019 Mar 12;14(3):e0213540. doi: 10.1371/journal.pone.0213540

Fig 6. Reduced salt tolerance in transgenic plants overexpressing GhWRKY68.

Fig 6

(A) Seed germination on 1/2 MS medium containing different concentrations of NaCl. (B) Germination rates of the WT and OE lines under 0 or 200 mM NaCl treatment conditions. Germination was scored daily. (C) Representative phenotypes of 8-week-old WT and OE plants grown in soil before and after 200 mM NaCl treatment for 1 month. (D) Survival rates of WT and OE plants after 200 mM NaCl treatment. (E) Representative phenotypes of the detached leaves of WT and OE plants treated with 200 mM NaCl. (F) Quantification of chlorophyll content. The values represent the chlorophyll content in the salt-treated plants relative to that of the untreated plants. BD, before drought treatment. The data presented are the means ± SE of three independent experiments (n = 3). Different letters above the columns in (D) and (F) indicate significant differences (P < 0.05) according to Duncan’s multiple range test performed using SAS version 9.1 software.