Table 2.
Cell expansion and culture duration of chicken and duck PGCs.
| Culture medium | Type of PGCs | Number of wells with cell expansion1/total number of wells2 (%) | Culture duration (days)3 |
|---|---|---|---|
| FAcs | CcPGCs | 6/10 (60.0) | >2503 |
| CgPGCs | 7/10 (70.0) | >2504 | |
| MDcPGCs | 2/32 (6.3) | 52.0 ± 6.0 | |
| MDgPGCs | 26/32 (81.3) | 5.6 ± 0.2 | |
| FAot | CgPGCs | 7/10 (70.0) | >2504 |
| MDgPGCs | 22/24 (91.7) | 8.9 ± 0.4 | |
| PDgPGCs | 13/24 (54.2) | 4.8 ± 0.2 | |
| MUDgPGCs | 21/24 (87.5) | 8.3 ± 0.4 |
1A well with cell expansion was defined as a culture well containing more than 1 × 105 expanded cells.
2PGCs isolated from each avian embryo were seeded in one separate well.
3The day on which cells stopped proliferating. Data are presented as mean ± SEM.
4CcPGCs and CgPGCs proliferated indefinitely in both types of media.