Table 1. Key Cas9 circular permutants.
Nomenclature and local sequence of select Cas9 circular permutants (Cas9-CPs). The superscript in the name indicates the original amino acid (AA) in Streptococcus pyogenes Cas9 that now serves as the new N-terminus.
| Name | Domain at CP site | Original seq at CP site | New start site (AA) |
|---|---|---|---|
| Cas9-CP181 | Helical-II | …PDNSD∣VDKLF… | 181 |
| Cas9-CP199 | Helical-II | …QLFEE∣NPINA… | 199 |
| Cas9-CP230 | Helical-II | …LIAQL∣PGEKK… | 230 |
| Cas9-CP270 | Helical-II | …QLSKD∣TYDDD… | 270 |
| Cas9-CP310 | Helical-II | …ILRVN∣TEITK… | 310 |
| Cas9-CP1010 | RuvC-III | …ESEFV∣YGDYK… | 1010 |
| Cas9-CP1016 | RuvC-III | …GDYKV∣YDVRK… | 1016 |
| Cas9-CP1023 | RuvC-III | …VRKMI∣AKSEQ… | 1023 |
| Cas9-CP1029 | RuvC-III | …KSEQE∣IGKAT… | 1029 |
| Cas9-CP1041 | RuvC-III | …YFFYS∣NIMNF… | 1041 |
| Cas9-CP1247 | CTD | …YEKLK∣GSPED… | 1247 |
| Cas9-CP1249 | CTD | …KLKGS∣PEDNE… | 1249 |
| Cas9-CP1282 | CTD | …SKRVI∣LADAN… | 1282 |