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. Author manuscript; available in PMC: 2019 Mar 12.
Published in final edited form as: Audiol Neurootol. 2017 Jan 10;21(6):356–364. doi: 10.1159/000449239

Figure 1: Schematic of surgical steps for round window (RW) injection in mice.

Figure 1:

A postauricular incision is made. The facial nerve is identified, and the bulla is exposed. 1) Bullostomy: An opening in the bulla is made, visualizing the stapedial artery and RW membrane. 2) RW puncture: The RW membrane is punctured with a glass micropipette. 3) RW injection: The glass micropipette is used to inject fluid (PBS with 5% glycerol) through the RW into the cochlea.