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. 2019 Feb 7;43(4):1657–1668. doi: 10.3892/ijmm.2019.4092

Figure 1.

Figure 1

Interleukin (IL)-22 is dynamically expressed during intestinal inflammation. (A) The expression of IL-22RA1 and IL-10RB in different cell types was detected by RT-qPCR. IECs-SI indicates the intestinal epithelium cells from small intestine, immune cells containing macrophages, monocytes, CD4+ T cells and dendritic cells (DCs). (B) Immunohistochemical staining of paraffin-emdedded sections of the small intestine and colon from wild-type (WT) mice by IL-22RA1 antibody (n≥3). Scale bar, 100 μm. The inset is the magnification of one representative crypt and the black arrowhead points to the intestinal stem cell (ISC). (C) H&E staining of colon from WT mice at different time points during dextran sodium sulfate (DSS)-induced inflammatory bowel disease (IBD). Scale bar, 100 μm. (D) The mRNA expression of IL-22 in colon during DSS-induced IBD in the animal model was determined by RT-qPCR. (E) The secreted IL-22 in the colon during DSS-induced IBD in the animal model was measured by ELISA. (F) H&E staining of the intestine from WT mice on days 0 and 3 after X-ray radiation. Scale bar, 100 μm. (G) The mRNA expression of IL-22 in the intestine was examined by RT-qPCR in the X-ray model. Data are the means ± SEM; *P<0.05, **P<0.01, and ***P<0.001 vs. day 0; analyzed by one-way ANOVA (n=5).