Expression of proteins of the RAS in the left myocardium as determined by (A and B) WB analysis and (C) immunohistochemistry (scale bar, 100 µm; black arrows indicate expression of the Mas receptor). (D and E) Expression of members of the RAS in the right myocardium as determined by WB analysis. Vertical lines in the western blot indicate non-adjacent bands from the same gel. Values are expressed as the mean ± standard deviation, N=5 pigs per group. *P<0.05; **P<0.01; ***P<0.001 vs. control group; #P<0.05; ##P<0.01; ###P<0.001 vs. the APE-CA group; &&&P<0.001 vs. the ROSC-SA group; APE-CA, acute pulmonary embolism with cardiac arrest; ROSC, return of the spontaneous circulation; SA, saline; ACE, angiotensin-converting enzyme; RAS, renin-angiotensin system; IHC, immunohistochemistry; AT1, angiotensin II receptor type 1; WB, western blot.