Table IV.
Statistical analysis of functional expression of OPN and MCP-1 in rat kidneys following 8 weeks of treatment.
| Group | OPN
|
MCP-1
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| − | ± | + | ++ | +++ | − | ± | + | ++ | +++ | |
| Control | 0 | 4 | 2 | 0 | 0 | 0 | 5 | 1 | 0 | 0 |
| EG | 0 | 0 | 1 | 5a,b | 0 | 0 | 0 | 2 | 4a,b | 0 |
| EG + metformin | 0 | 4 | 1 | 1 | 0 | 0 | 2 | 3 | 1 | 0 |
Summary data from quantitative analysis of the immunohistochemical distribution of OPN and MCP-1 in rat kidneys following 8 weeks of treatment via the Wilcoxon rank-sum test (n=6).
P<0.05, EG, vs. control;
P<0.05, EG, vs. EG + metformin. Expression levels of OPN and MCP-1 were carefully evaluated. −, negative expression; +/−, positive rate of expression <25%; +, positive rate of expression <50%; ++, positive rate of expression <75%; and +++, positive rate of expression >75%. The numbers listed in this table represent the total number of animals with a negative or positive result in each group following 8 weeks of treatment. OPN, osteopontin; MCP-1, monocyte chemoattractant protein 1; EG, ethylene glycol.