Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | antibody to Bcl-XL (Rabbit polyclonal) | PMID: 14681679 | (1:10,000), human, mouse reactivity | |
| Antibody | antibody to Beta-actin (Mouse monoclonal) | Abgent | Cat. #: 8H10D10 | (1:5000), human, rat, mouse reactivity |
| Antibody | Donkey anti-rabbit (polyclonal) | Jackson Immuno Research Laboratories | Cat. #: 711-035-150 | (1:10,000) |
| Antibody | Donkey anti-mouse (polyclonal) | Jackson Immuno Research Laboratories | Cat. #: 711-035-152 | (1:10,000) |
| Cell line (H.sapiens) | MCF-7 | PMID: 3790748 | Dr. Ronald N. Buick (University of Toronto) | |
| Cell line (M. musculus) | Baby Mouse Kidney(BMK)-DKO (Bax and Bak knockout) cells | PMID: 11836241 | Dr. Eileen White (Rutgers University) | |
| Chemical compound, drug | Navitoclax; ABT-263 | Selleckchem | Cat. #: S1001 | in DMSO |
| Chemical compound, drug | A-1155463 | Chemietek | Cat #: CT-A115 | in DMSO |
| Chemical compound, drug | A-1331852 | Chemietek | Cat. #: CT-A115 | in DMSO |
| Chemical compound, drug | S-63845 | Chemietek | Cat. #: 1799633-27-4 | in DMSO |
| Chemical compound, drug | Venetoclax; ABT-199 | Chemietek | Cat. #: CT-A199 | in DMSO |
| Chemical compound, drug | TMRE | ThermoFisher Scientific | Cat. #: T669 | |
| Chemical compound, drug | DRAQ5 | Biostatus, UK | Cat. #: DR05500 | |
| Chemical compound, drug | MitoTracker Red | ThermoFisher Scientific | Cat. #: M22425 | |
| Chemical compound, drug | MitoTracker Green | ThermoFisher Scientific | Cat. #: M7514 | |
| Chemical compound, drug | Alexa 647-maleimide | ThermoFisher Scientific, Molecular probes | Cat. #: A20347 | |
| Chemical compound, drug | Alexa568-maleimide | ThermoFisher Scientific, Molecular probes | Cat. #. A20341 | |
| Chemical compound, drug | NBD | Molecular Probes | Cat. #: D-2004 | |
| Chemical compound, drug | PC (L-α-phosphatidylcholine) | Avanti Polar Lipids | Cat. #:840051C | for making liposomes, used 48% PC |
| Chemical compound, drug | DOPS (1,2-dioleoyl-sn-glycero-3-phospho-L-serine) | Avanti Polar Lipids | Cat. #: 840035C | for making liposomes, used 10% DOPS |
| Chemical compound, drug | PI (L-α-phosphatidylinositol) | Avanti Polar Lipids | Cat. #:840042C | for making liposomes, used 10% PI |
| Chemical compound, drug | PE (L-α-phosphatidylethanolamine) | Avanti Polar Lipids | Cat. #: 841118C | for making liposomes, used 28% PE |
| Chemical compound, drug | TOCL, (18:1 Cardiolipin) | Avanti Polar Lipids | Cat. #: 710335C | for making liposomes, used 4% TOCL |
| Commercial assay or kit | Fugene HD | Promega | Cat. #: E2311 | |
| Commercial assay or kit | TransIT-X2 | Mirus | Cat. #: Mir 6003 | |
| Gene (H. sapiens) | Bax | PMID: 14522999, | GI: L22473.1 | For recombinant protein |
| Gene (H. sapiens) | Bcl-XL | PMID: 18547146 | GI: Z23115.1 | For recombinant protein |
| Gene (H. sapiens) | Bcl-2 | PMID: 22464442 | GI: M14745.1 | For expression of CBcl-2 in cells |
| Gene (H. sapiens) | Bad | PMID: 22464442 | GI: AB451254.1 | For expression of VBad in cells |
| Gene (H. sapiens) | Bcl-XL | PMID: 22464442 | GI: NM_138578.3 | For expression of CBcl-XL in cells |
| Gene (M. musculus) | Bid | PMID: 16642033, PMID: 19062087 |
GI: NM_007544.4 | For recombinant protein |
| Gene (M. musculus) | BimL | this paper | GI: AAD26594.1 | This lab, plasmid # 2187, for recombinant BimL purification |
| Gene (M. musculus) |
tBid | PMID: 22464442 | GI: NM_007544.4 | for expression of VtBid in cells |
| Gene (M. musculus) | BimEL | PMID: 22464442 | GI: XM_006498614.3 | for expression of VBimEL in cells |
| Other | Cell Carrier-384, Ultra | PerkinElmer | Cat. #: 6057300 | for live cell imaging |
| Other | Non-binding surface, 96-well plate, black with clear bottom | Corning | Cat. #: 3881 | For recombinant protein and liposome assays critical to use non-binding plate |
| Other | Opera Phenix | PerkinElmer | Cat. #: HH14000000 | |
| Other | ISS-Alba | PMID: 25631031 | Custom built by ISS for DWA lab | |
| Software, algorithm | GraphPad Prism | San Diego, California | Version 6 | Scientific graphing program, used to perform statistical analysis |
| Software, algorithm | ImageJ | PMID: 17936939 | MBF - ImageJ for microscopy, Dr. Tony Collins (McMaster University) | FLIM-FRET analysis Macro, in this paper: https://github.com/DWALab/Liu_et_al_2018_eLife |
| Software, algorithm | CellProfiler | PMID: 17269487 | Colocalization analysis, in this paper: https://github.com/DWALab/Liu_et_al_2018_eLife | |
| Transfected Construct | mVenus-pEGFP-C1 | other | GI: KU341334.1 | Dr. Ray Truant (McMaster University). Backbone EGFP-C1 (Clonetech) |
| Transfected Construct | mCerulean3-pEGFP-C1 | PMID: 21479270 | Dr. Mark A Rizzo (University of Maryland). Backbone EGFP-C1 (Clonetech) | |
| Transfected Construct | pSPUTK | Stratagene Santa Clara CA | Cat. #: CB4278654 | Cotransfected to reduce overexpression in live cells |