Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus, NOD/SCID, male) |
NOD/SCID | Vital River | Strain code: 406; RRID:IMSR_CRL:394 | |
| Cell line (Homo sapiens, male) |
LAPC4 | doi:10.1038/nm0497-402 | RRID:CVCL_4744 | shared by Dr. Dean G Tang, MD Anderson Cancer Center and Dr. Charles L Sawyers, Memorial Sloan Kettering Cancer Center |
| Cell line (H. sapiens, male) |
DU145 | ATCC | cat#:HTB-81; RRID:CVCL_0105 | |
| Cell line (H. sapiens, male) |
HT-1080 | ATCC | cat#:CCL-121; RRID:CVCL_0317 | |
| Cell line (H. sapiens) |
HEK293T | ATCC | cat#:CRL-3216; RRID:CVCL_0063 | |
| Chemical compound, drug |
testosterone propionate | Aladdin | CAS:57-85-2 | lot# L1401028 |
| Other | Matrigel | BD Biosciences | cat#:354248 | |
| Antibody | FITC-conjugated monoclonal anti-CD44 | BD Biosciences | cat#:555478; clone:G44-26; RRID:AB_395870 | 1:13 dilution |
| Antibody | FITC-conjugated monoclonal mouse IgG2b,κ isotype control | BD Biosciences | cat#:555742; clone:27–35; RRID:AB_396085 | 1:13 dilution |
| Antibody | rabbit anti-CD44 | AbCam | cat#:ab51037; clone:EPR1013Y; RRID:AB_868936 | 1:1000 dilution |
| Antibody | mouse anti-ß-actin | Cell Signaling Technology | cat#:3700; clone:8H10D10; RRID:AB_2242334 | 1:1000 dilution |
| Antibody | HRP-conjugated goat anti-rabbit | Cell Signaling Technology | cat#:7074; RRID:AB_2099233 | 1:2000 dilution |
| Antibody | HRP-conjugated horse anti-mouse | Cell Signaling Technology | cat#:7076; RRID:AB_330924 | 1:5000 dilution |
| Commercial assay, kit |
MACS Lineage Cell Depletion Kit | Miltenyi Biotech | cat#:130-090-858 | |
| Recombinant DNA reagent |
psPAX2 | Crown Biosciences, Inc | RRID:Addgene_12260 | |
| Recombinant DNA reagent |
pMD2.G | Crown Biosciences, Inc | RRID:Addgene_12259 | |
| Recombinant DNA reagent |
pMDLg/pRRE | Wuhan Miaoling Bioscience and Technology Co. |
cat# P0685; RRID:Addgene_12251 | |
| Recombinant DNA reagent |
pre-mir-34a | System Biosciences | cat# PMIRH34aPA-1 | |
| Recombinant DNA reagent |
scramble non-targeting pre-miRNA | System Biosciences | cat# PMIRH000PA-1 | |
| Recombinant DNA reagent |
pMIR-CD44-3'UTR | doi:10.1038/nm.2284 | shared by Dr. Dean G Tang, MD Anderson Cancer Center | |
| Recombinant DNA reagent |
pMIR-CD44-M1M2-3'UTR | doi:10.1038/nm.2284 | shared by Dr. Dean G Tang, MD Anderson Cancer Center | |
| Recombinant DNA reagent |
phRL-CMV | doi:10.1038/nm.2284 | shared by Dr. Dean G Tang, MD Anderson Cancer Center | |
| Sequence-based reagent |
copGFP | doi:10.1111/j.1745-7270.2008.00448.x | Forward: 5'-AGGACAGCGTGATCTTCACC-3'; Reverse: 5'-CTTGAAGTGCATGTGGCTGT-3' | |
| Sequence-based reagent |
miR-34a miRNA TaqMan assay kit | Applied Biosystems | assayID:000426 | |
| Sequence-based reagent |
has-let-7b miRNA TaqMan assay kit | Applied Biosystems | assayID:000378 | |
| Sequence-based reagent |
miR-103 miRNA TaqMan assay kit | Applied Biosystems | assayID:000439 | |
| Sequence-based reagent |
has-miR-34a-5p | Thermo Fisher Scientific | assayID:MC11030 | |
| Sequence-based reagent |
negative control #1 | Thermo Fisher Scientific | cat#:4464058 | |
| Software, algorithm |
GeneMapper | Applied Biosystems | RRID:SCR_014290 | version 4.0 |
| Software, algorithm |
Studylog Systems | Studylog | RRID:SCR_016682 | version 3.1.399.19 |
| Software, algorithm | FACSDiva | BD Biosciences | RRID:SCR_001456 | version 6.1.3 |
| Software, algorithm | MxPro QPCR | Stratagene | RRID:SCR_016375 | version 4.1 |
| Software, algorithm | ImageJ | doi:10.1038/nmeth.2089 | RRID:SCR_003070 | version 1.50a |
| Software, algorithm | EnVision | Perkin Elmer | RRID:SCR_016681 | version 1.12 |
| Software, algorithm | R Project for statistical computing | https://www.r-project.org | RRID:SCR_001905 | version 3.5.1 |