Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line | CEM-T4 | NIH AIDS Reagent Program | Cat. #: 117 | |
| Antibody | Mouse monoclonal BV421-conjugated anti-CD4 | BioLegend | Cat. #: 317434 | Flow cytometry |
| Antibody | Mouse monoclonal PE-conjugated anti-CD4 | BD Biosciences | Cat. #: 561843 | Flow cytometry |
| Antibody | Mouse monoclonal PE-conjugated anti-tetherin | BioLegend | Cat. #: 348405 | Flow cytometry |
| Antibody | Mouse monoclonal AF647-conjugated anti-LNGFR | BioLegend | Cat. #: 345114 | Flow cytometry |
| Antibody | Mouse monoclonal FITC-conjugated anti-LNGFR | BioLegend | Cat. #: 345103 | Flow cytometry |
| Antibody | Rabbit monoclonal anti-PPP2R5D | Abcam | Cat. #: ab188323 | Immunoblot |
| Antibody | Mouse monoclonal anti-HIV-1 Vif | NIH AIDS Reagent Program | Cat. #: 6459 | Immunoblot |
| Antibody | Mouse monoclonal anti-p24 | Abcam | Cat. #: ab9071 | Immunoblot |
| Antibody | Mouse monoclonal anti-HIV-1 Nef | NIH AIDS Reagent Program | Cat. #: 3689 | Immunoblot |
| Antibody | Rabbit monoclonal anti-PTPN22 (D6D1H) | Cell Signalling Technology | Cat. #: 14693 | Immunoblot |
| Antibody | Mouse monoclonal anti-ARID5A | GeneTex | Cat. #: GTX631940 | Immunoblot |
| Antibody | Rabbit polyclonal anti-FMR1 (FMRP) | Cell Signalling Technology | Cat. #: 4317 | Immunoblot |
| Antibody | Rabbit polyclonal anti-DPH7 | Atlas Antibodies | Cat. #: HPA022911 | Immunoblot |
| Antibody | Mouse monoclonal anti-α-tubulin | Cell Signalling Technology | Cat. #: 3873 | Immunoblot |
| Antibody | Mouse monoclonal anti-β-actin | Sigma | Cat. #: A5316 | Immunoblot |
| Antibody | Rabbit polyclonal anti-total AURKB | Cell Signalling Technology | Cat. #: 3094 | Immunoblot |
| Antibody | Rabbit monoclonal anti-phospho-AURK | Cell Signalling Technology | Cat. #: 2914 | Immunoblot |
| Recombinant DNA reagent | HIV-AFMACS | This paper | GenBank: MK435310 | pNL4-3-ΔEnv-Nef-P2A-SBP-ΔLNGFR proviral construct (see Materials and methods) |
| Recombinant DNA reagent | pCtrl | (Matheson et al., 2014) | Not applicable | pHRSIN-SE-P2A-SBP-ΔLNGFR-W expression vector |
| Recombinant DNA reagent | pVif | This paper | Not applicable | pHRSIN-SE-P2A-Vif-hu-W expression vector (see Materials and methods) |
| Recombinant DNA reagent | pSBP-ΔLNGFR | This paper | Not applicable | pHRSIN-S-P2A-SBP-ΔLNGFR-W expression vector (see Materials and methods) |
| Recombinant DNA reagent | pTat/SBP-ΔLNGFR | This paper | Not applicable | pLTR-Tat-P2A-SBP-ΔLNGFR expression vector (see Materials and methods) |
| Commercial assay or kit | Dynabeads Untouched Human CD4 T Cells kit | Invitrogen | Cat. #: 11346D | |
| Commercial assay or kit | Dynabeads Human T-Activator CD3/CD28 | Gibco | Cat. #: 11132D | |
| Commercial assay or kit | Dynabeads Biotin Binder | Invitrogen | Cat. #: 11047 | |
| Commercial assay or kit | iST-NHS Sample Preparation Kit | PreOmics | Cat. #: P.O.00030 | |
| Commercial assay or kit | S-Trap micro MS Sample Preparation Kit | Protifi | Cat. #: C02-micro | |
| Commercial assay or kit | TMT10plex Isobaric Label Reagent Set | Thermo Scientific | Cat. #: 90110 | |
| Chemical compound, drug | Lympholyte-H | Cedarlane Laboratories | Cat. #: CL5020 | |
| Chemical compound, drug | IL-2 | PeproTech | Cat. #: 200–02 | Recombinant human IL-2 |
| Chemical compound, drug | Lenti-X Concentrator | Clontech | Cat. #: 631232 | |
| Software, algorithm | Proteome Discoverer 2.1 | Thermo Scientific | RRID: SCR_014477 | |
| Software, algorithm | DAVID 6.8 | (Huang et al., 2009a;Huang et al., 2009b) | RRID: SCR_001881 | https://david.ncifcrf.gov/ |
| Software, algorithm | Cytoscape 3.6.1 | (Shannon et al., 2003) | RRID: SCR_003032 | http://cytoscape.org/ |
| Software, algorithm | Enrichment Map 3.1.0 Cystoscape plugin | (Merico et al., 2010) | RRID:SCR_016052 | http://baderlab.org/Software/EnrichmentMap |
| Software, algorithm | Cluster 3.0 | (de Hoon et al., 2004) | RRID:SCR_013505 | http://bonsai.hgc.jp/~mdehoon/software/cluster/software.htm |
| Software, algorithm | Java TreeView 1.1.6r4 | (Saldanha, 2004) | RRID:SCR_013503 | http://jtreeview.sourceforge.net |