Figure 3. Altered energy metabolism of rcd1.

Deduced metabolic fluxes in light- and dark- adapted Col-0 and rcd1 rosettes were assessed by fractionation of the extracts of leaves treated with [U-¹⁴C] glucose. Increased respiration flux and higher amount of total metabolized glucose (Figure 3—source data 1) in rcd1 suggest a more active glycolytic pathway. Higher cell wall metabolic flux in rcd1 provided indirect support of increased operation of the oxidative pentose phosphate pathway which is required for generating pentoses used in cell wall biosynthesis (Ap Rees, 1978). Mean ±SE are presented. Asterisks indicate values significantly different from the wild type, **P value < 0.01, *P value < 0.05, Student’s t-test. Source data and statistics are presented in Figure 3—source data 2.