Table 1. Overview of the immunoprecipitation results.
Selected proteins identified in ANAC013-GFP and RCD1-3xVenus pull-down assays. Ratio vs. Col-0 and the P-value were obtained by Perseus statistical analysis from the three repeats for each genotype used. Bold text indicates baits. The peptide coverage for selected proteins as well as full lists of identified proteins are presented in Figure 7—source datas 1 and 2.
| Ratio vs. Col-0 | P-value | Unique peptides | Gene | Name | Stickiness |
|---|---|---|---|---|---|
| ANAC013-GFP pull-down | |||||
| 50966 | 7.09 × 10−7 | 29 | AT1G32870 | ANAC013 | |
| 22149 | 3.41 × 10−8 | 25 | GFP | ||
| 10097 | 3.67 × 10−6 | 37 | AT1G32230 | RCD1 | 1.00% |
| 110 | 1.67 × 10−6 | 8 | AT2G35510 | SRO1 | 1.00% |
| 74 | 1.09 × 10−9 | 4 | AT1G34190 | ANAC017 | 1.00% |
| RCD1-3xVenus pull-down | |||||
| 7593 | 0.000454 | 35 | AT1G32230 | RCD1 | |
| 1292 | 0.006746 | 10 | YFP | ||
| 108 | 5.48 × 10−8 | 2 | AT1G34190 | ANAC017 | 1.00% |