| Antibodies |
|
|
| Mouse anti-hNotch1 (extracellular domain) |
BioLegend |
Cat# 352014; RRID:AB_10899408 |
| Rabbit anti-hN1ICD (V1744) monoclonal
[D3B8] |
Cell Signaling Technology |
Cat# 4147; RRID:AB_2153348 |
| Mouse anti-mGAPDH [6C5] |
Abcam |
Cat# ab8245; RRID:AB_2107448 |
| Chemicals, Peptides, and Recombinant
Proteins |
|
|
| Human DII1ext-IgG |
Sprinzak et
al., 2010 |
N/A |
| Mouse N1ext-mFc |
R&D systems |
Cat# 5267-TK |
| Hamster Fibronectin |
Oxford Biomedical Research |
Cat# CT30 |
| DAPT |
Sigma Aldrich |
Cat# D5942 |
| 4-epi tetracycline Hydrochloride |
Sigma Aldrich |
Cat# 37918 |
| Critical Commercial Assays |
|
|
| RNeasy mini kit for RNA extraction |
QIAGEN |
Cat# 74106 |
| iScript cDNA synthesis kit |
Bio-Rad |
Cat# 1708890 |
| iQ SYBR Green Supermix |
Bio-Rad |
Cat# 1708880 |
| DNA HCR kit |
Molecular Instruments |
N/A |
| Deposited Data |
|
|
| Raw and analyzed RNaseq data |
This paper |
GSE72847 |
| Experimental Models: Cell Lines |
|
|
| CHO-K1 |
ATCC |
Cat# CCL-61; RRID:CVCL_0214 |
| CHO TREx |
Invitrogen |
RRID:CVCL_D586 |
| C2C12 |
ATCC |
Cat# CRL-1772; RRID:CVCL_0188 |
| CHO-K1-TREx + pcDNA3-CMV-hN1ECD-Gal4esn +
pEV-UAS-H2B-3xCitrine |
This paper |
N/A |
| CHO-K1-TREx + pcDNA3-CMV-hN1ECD-Gal4esn +
pEV-UAS-H2B-3xCitrine + pGK-H2B-mCherry |
This paper |
N/A |
| CHO-K1-TREx +
pcDNA5-CMV-TO-rDll1-T2A-H2B-mCherry |
This paper |
N/A |
| CHO-K1-TREx +
pcDNA5-CMV-TO-rDll1-T2A-H2BmCherry + pLenti-CAG-H2B-Cerulean |
This paper |
N/A |
| CHO-K1-TREx +
pcDNA5-CMV-TO-hDll4-T2A-H2B-mCherry |
This paper |
N/A |
| CHO-K1-TREx + pb-CMV-TO-hDll4-T2A-H2BmCherry +
pb-CMV-H2B-Cerulean |
This paper |
N/A |
| CHO-K1-TREx + pb-CMV-TO-Gal4esn-T2A-H2BmCh +
UAS-H2B-Citrine |
This paper |
N/A |
| CHO-K1-TREx + pEF-hN1ECD-Gal4esn + UAS-H2B-
3xCitrine-3′Hes1UTR |
This paper |
N/A |
| CHO-K1-TREx +
pcDNA5-CMV-TO-rDll1ECD-
Dll4ICD-T2A-H2B-mCherry |
This paper |
N/A |
| CHO-K1-TREx +
pcDNA5-CMV-TO-hDll4ECD-
Dll1ICD-T2A-H2B-mCherry |
This paper |
N/A |
| CHO-K1-TREx + pcDNA5-CMV-TO-rDll1-FLAG |
This paper |
N/A |
| CHO-K1-TREx + pcDNA5-CMV-TO-hDll4-FLAG |
This paper |
N/A |
| CHO-K1-TREx + pb-CMV-hN1-T2A-H2B-mCherry |
This paper |
N/A |
| CHO-K1-TREx + pcDNA5-CMV-TO-
hDll4ECD-Dll1ICD -FLAG |
This paper |
N/A |
| C2C12 + pb/CMV7-hN1-myc-T2A-H2B-mCherry |
This paper |
N/A |
| C2C12 +
pb-CMV-TO-hN1ΔECD-T2A-H2B-mCherry |
This paper |
N/A |
| C2C12 + pb/hNECD-Gal4esn-ANK-T2A-H2B-Cer +
pEV/UAS-dm-H2B-3xCit |
This paper |
N/A |
| C2C12 + pb/TO-hDll1-T2A-H2B-mCh-P2A-Hyg |
This paper |
N/A |
| C2C12 + pb/TO-hDll1-T2A-H2B-mCh-P2A-Hyg |
This paper |
N/A |
| Experimental Models: Organisms |
|
|
| Stage 12–13 chicken embryos |
This paper |
N/A |
| Recombinant DNA |
|
|
| pcDNA3/CMV-hN1ECD-Gal4esn |
Sprinzak et
al., 2010 |
N/A |
| pEV/UAS-H2B-3xCitrine |
LeBon et al.,
2014 |
N/A |
| pcDNA5/CMV-TO-rDll1-T2A-H2B-mCherry |
This paper |
N/A |
| pcDNA5/CMV-TO-hDll4-T2A-H2B- mCherry |
This paper |
N/A |
| pb/CMV-TO-Gal4esn-T2A-H2B- mCherry |
This paper |
N/A |
| pEV/UAS-H2B-3xCitrine-3′ Hes1 UTR |
This paper |
N/A |
| pcDNA5/CMV-TO-Dll1ECD-Dll4ICD
|
This paper |
N/A |
| pcDNA5/CMV-TO-Dll4ECD-Dll1ICD
|
This paper |
N/A |
| pcDNA5/CMV-TO-Dll1-FLAG |
This paper |
N/A |
| pcDNA5/CMV-TO-Dll4ECD-Dll1ICD-FLAG |
This paper |
N/A |
| pcDNA5/CMV-TO-Dll4-FLAG |
This paper |
N/A |
| pb/CMV-TO-hN1DECD-T2A-H2B- mCherry |
This paper |
N/A |
| pb/CMV7-hN1-myc-T2A-H2B- mCherry |
This paper |
N/A |
| pCI/CAGG-rDll1-T2A-EGFP |
This paper |
N/A |
| pCI/CAGG-hDll4-T2A-EGFP |
This paper |
N/A |
| pb/CMV-TO-hDll1-T2A-H2B- mCherry |
This paper |
N/A |
| pb/CMV-TO-hDll4-T2A-H2B- mCherry |
This paper |
N/A |
| Oligonucleotides |
|
|
| siRNA targeting mouse Notch2 5′-UGAACUU
GCAGGAUGGGUGAAGGUC-3′ |
Invitrogen |
N/A |
| mouse Hes1 primer set 1
(Figure 3C) - Forward,
5′-CAACACGACACCGGACAAAC-3′ |
IDT DNA |
N/A |
| mouse Hes1 primer set 1
(Figure 3C) - Reverse,
AAGAATAAATGAAAGTCTAAGCCAA-3′ |
IDT DNA |
N/A |
| Mouse Hes1 primer set 2
(Figure 3D, S4, 5) – Forward,
5′-AAGAATAAATGAAAGTCTAAGCCAA-3′ |
IDT DNA |
N/A |
| Mouse Hes1 primer set 2
(Figure 3D, S4, 5) – Reverse,
5′-TTCTTGCCCTTCGCCTCTTC-3′ |
IDT DNA |
N/A |
| mouse Hey1 primers –
Forward, 5′-GCCGAAGTTG CCCGTTATCT-3′ |
IDT DNA |
N/A |
| mouse Hey1 primers –
Reverse, 5′-CGCTGGGATG CGTAGTTGTT-3′ |
IDT DNA |
N/A |
| mouse HeyL primers –
Forward, 5′-GAGCTGAC TTCCCACAACCA-3′ |
IDT DNA |
N/A |
| mouse HeyL primers –
Reverse, 5′-GAGAGG TGCCTTTGCGTAGA-3′ |
IDT DNA |
N/A |
| mouse SdhA primers - Forward,
5′-AGTGGGCT GTCTTCCTTAAC-3′ |
IDT DNA |
N/A |
| mouse SdhA primers - Reverse,
5′-GGATTGCTTCT GTTTGCTTGG-3′ |
IDT DNA |
N/A |
| Software and Algorithms |
|
|
| Segmentation, tracking, and fluorescence
analysis software |
This paper |
https://github.com/nnandago/cell2017-segtrack |
