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. Author manuscript; available in PMC: 2020 Mar 22.
Published in final edited form as: Vaccine. 2019 Feb 20;37(13):1807–1818. doi: 10.1016/j.vaccine.2019.02.022

Figure 1: Generation of specific humoral immune response against Tp0435 protein using B. burgdorferi expressing this T. pallidum protein as immunogen in Balb/c mice.

Figure 1:

Mice were immunized with 100 μl of non-infectious, bioluminescent B. burgdorferi strains B31HP- and B314-expressing Tp0435 mixed with an equal volume of 30 mg/ml D-luciferin substrate for B. burgdorferi codon-optimized luciferase encoded by the pJSB175-tp0435 plasmid. (A) Bioluminescence was observed within 10 mins of injection of the inoculum. (B) Loss of bioluminescence was detected within 24h, after delivering additional D-luciferin substrate at the cell injection site. (C) After a total of three immunizations, antibody titer against recombinant Tp0435 protein was determined by ELISA. B31HP-Tp0435: sera from mice immunized with Tp0435-expressing B31HP strain; B314-Tp0435: sera from mice immunized with Tp0435-expressing B314 strain. An antiserum against rTp0435 previously generated in mice using Freund’s adjuvant [33] was included as a control antiserum.