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. 2019 Feb 11;77(1):ftz007. doi: 10.1093/femspd/ftz007

Figure 2.

Figure 2.

Increased EBI3 accumulates in virulent M. tb-treated murine macrophages. (A) and (B) Murine peritoneal macrophages were stimulated with iH37Rv or iBCG for 2 h, 6 h, 18 h and 24 h. (A) Extracellular EBI3 level in cell culture supernatant was determined by ELISA. The data are shown as mean ± SD (n = 3). (B) Intracellular EBI3 was determined by immunoblot analysis. The iH37Rv/iBCG-treated cell pellets were washed with PBS, and employed to determine the intracellular EBI3. (C) Peritoneal macrophages and (D) RAW 264.7 cells were stimulated with iH37Rv or iBCG for 6 h. Intracellular EBI3 levels were determined by confocal microscopy.