FIG 4.
Correlation between the activity of combinations of different alleles of IRC7 and the release of 4-MMP (filled circles) and 3-MH (empty circles) in SGJ-thiol medium by diploid strains exhibiting these alleles. Activity was evaluated in protein extracts in triplicate from cells overexpressing different alleles of IRC7 using Cys-4-MMP as the substrate. The IRC7L allele was given an arbitrary value of 100, and diploid strains with the IRC7L IRC7L genotype were given a value of 200. The correlation coefficient (R2) for 4-MMP and 3-MH was found to be 0.95 and 0.90, respectively.