TABLE 7.
Strains and plasmids generated in this study
| IRC7 allele (donor strain) | Mutation(s) | Strainb | Plasmidc |
|---|---|---|---|
| IRC7S (BY4742) | 1023_1060dela | AWRI1631 IRC7S | pHVX2-IRC7S |
| IRC7L (AWRI838) | AWRI1631 IRC7L | pHVX2-IRC7L | |
| IRC7L T185A (AWRI1688) | 553A→G | AWRI1631 IRC7L T185A | pHVX2-IRC7L T185A |
| IRC7L T185A K43R (AWRI1493) | 128A→G, 553A→G | AWRI1631 IRC7L T185A K43R | pHVX2-IRC7L T185A K43R |
| IRC7L T185A P146R (AWRI722) | 437C→G, 553A→G | AWRI1631 IRC7L T185A P146R | pHVX2-IRC7L T185A P146R |
| IRC7L T185A G253R (AWRI1899) | 553A→G, 757G→A | AWRI1631 IRC7L T185A G253R | pHVX2-IRC7L T185A G253R |
| IRC7L T185A G321D (AWRI723) | 553A→G, 962G→A | AWRI1631 IRC7L T185A G321D | pHVX2-IRC7L T185A G321D |
| IRC7L T185A E323G (AWRI935) | 553A→G, 968A→G | AWRI1631 IRC7L T185A E323G | pHVX2-IRC7L T185A E323G |
a
The deletion of 38 bp in the IRC7S allele results in a protein of 340 amino acids (Gly340*), truncated compared to the full-length allele (IRC7L), which encodes a protein of 400 amino acids.
b
IRC7 alleles from different donor strains were integrated at the IRC7 chromosomal location of the haploid wine strain AWRI1631.
c
The multicopy episomal plasmid pHVX2 (55) was used to overexpress IRC7 alleles from different donor strains, under the control of the constitutive PGK1 promoter.