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General properties of recombinant BlArafC and BlArafB. pNP-α-Araf was incubated with recombinant enzymes at 37°C. (A) Effect of pH on BlArafC. (B) Effect of pH on BlArafB. (C) Effect of temperature on BlArafC. (D) Effect of temperature on BlArafB. (E) Thermal stability of BlArafB at high temperatures. Error bars indicate SD (n = 3). For stability tests, enzymes were preincubated in each pH buffer for 16 h at 37°C or in sodium acetate buffer (pH 6.0) at each temperature for 1 h.
