Comparison of the BacV4V5-1, BacV6-21, HB, and HF183/BacR287 assay copy numbers (CN) in sewage samples. (A) Results from four assays showing CN in 20 sewage samples from Jones Island (JI) and South Shore (SS) wastewater treatment plants, Milwaukee, WI. (B) Results from four assays showing CN in 20 sewage samples from ten other U.S. cities, each tested at two different time points.