Cytokine responses in mice immunized intranasally with S. mitis. Samples were collected from the nasal wash, BALF, and sera from the immunized mice, and the levels of cytokines were measured using ELISA. The data are shown as means ± SD and were pooled from the results of two independent experiments with 4 mice in each group. Each symbol represents data from an individual mouse, and the horizontal bars are mean values for the groups. **, P < 0.01; ns, not significant (unpaired Student's t test).