FIG 5.

Production of cytokines by splenic CD4⁺ T cells after S. mitis immunization. IL-17A, IFN-γ, and IL-4 production in CD4⁺ T cells isolated from the spleen was analyzed by flow-cytometric intracellular-cytokine staining. Cells were gated on CD3⁺ CD4⁺ T cells as described in the legend to Fig. 4. Representative flow-cytometric images (left) and a summary of the percentages of cytokine-producing CD4⁺ T cells (right) are shown. The data are shown as means ± SD and were pooled from the results of two independent experiments with 4 mice in each group. Each symbol represents data from an individual mouse, and the horizontal bars are mean values for the groups; ns, not significant (unpaired Student's t test).