FIG 10.

Docking model of streptothricin and BaSatA. AutoDock Vina (31) was used to create a model of streptothricin (shown in yellow) docked to BaSatA. (A) Whole structure of BaSatA highlighting substituted residues. (B) Conserved aromatic residues Y149 (purple), F154 (green), and Y164 (blue) form a pocket around streptothricin, while residue D160 (orange) is found on the dimer interface. (C) Residue E137 (red) is close enough to the beta-lysine for a nucleophilic attack on the amine, suggesting E137 is an active-site acid. Residue Y164 (blue) is in close proximity to interact with streptothricin through hydrogen bonding.