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. 2019 Mar 6;85(6):e03029-18. doi: 10.1128/AEM.03029-18

FIG 2.

FIG 2

Variant B. subtilis satA alleles cannot confer streptothricin resistance in S. enterica. Strains carrying the wild type (B. subtilis satA+, circles), variant B. subtilis satA alleles, or empty vector (squares) were grown in glycerol (22 mM) minimal medium and challenged with 10 μM streptothricin either without inducer (closed symbols) (A and B) or with 200 μM l-(+)-arabinose (open symbols) (C and D). Names next to arrows represent the protein produced by each strain. The following strains were analyzed: JE22263 (ΔmetE2702 ara-9/pCV1; squares), JE22334 (ΔmetE2702 ara-9/pBsSATA1; circles), JE23884 (ΔmetE2702 ara-9/pBsSATA10; diamonds), JE23887 (ΔmetE2702 ara-9/pBsSATA11; triangles), JE23888 (ΔmetE2702 ara-9/pBsSATA12; hexagons), and JE23889 (ΔmetE2702 ara-9/pBsSATA13; downward triangles). Error bars represent one standard deviation. vector, pCV1 (27).