FIG 6.
B. subtilis SatAE129Q cannot confer streptothricin resistance in S. enterica. Strains carrying wild-type alleles (B. subtilis satA+, circles), variant B. subtilis satA alleles, or empty vector (squares) were grown in glycerol (22 mM) minimal medium, challenged with 10 μM streptothricin either without inducer (closed symbols) or with 250 μM l-(+)-arabinose (open symbols). The following strains were analyzed: JE22263 (ΔmetE2702 ara-9/pCV1; squares), JE22334 (ΔmetE2702 ara-9/pBsSATA1; circles), JE24032 (ΔmetE2702 ara-9/pBsSATA9; diamonds), and JE23657 (ΔmetE2702 ara-9/pBsSATA4; triangles). Error bars represent one standard deviation. vector, pCV1 (27).