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. 2019 Mar 6;85(6):e03029-18. doi: 10.1128/AEM.03029-18

TABLE 1.

Size exclusion chromatography behavior of B. subtilis and B. anthracis SatA variantsa

Variant Molecular mass (kDa) Oligomeric state (n)
Bacillus subtilis SatA
    BsSatA monomer (predicted) 23 1
    BsSatAWT 37 1.7
    BsSatAS84C 39 1.7
    BsSatAL126F 40 1.7
    BsSatAL128F 33 1.4
    BsSatAA137T 47 2
Bacillus anthracis SatA
    BaSatA monomer (predicted) 24.5 1
    BaSatAWT 50 2
    BaSatAY149G 56 2.3
    BaSatAY149A 55 2.2
    BaSatAY149S 55.5 2.3
    BaSatAY149L 49 2
    BaSatAY149F 59 2.4
    BaSatAY149W 55 2.2
    BaSatAF154G 50 2
    BaSatAF154L 56 2.3
    BaSatAF154Y 50 2
    BaSatAF154W 49 2
    BaSatAD160G 168 Aggregate
    BaSatAD160A 174 Aggregate
    BaSatAD160E 467 Aggregate
    BaSatAD160N 316 Aggregate
    BaSatAY164G 45 1.8
    BaSatAY164A 48 2
    BaSatAY164S 58 2
    BaSatAY164L 48.5 2
    BaSatAY164F 50 2
    BaSatAY164W 49 2
a

Calculated molecular masses and oligomeric states for H6-BsSatA, H6-BaSatA, and their variants are shown. Samples were applied to a Superose 12 10/300 size exclusion column, and retention data were compared to a calibration curve generated from standards to calculate molecular mass.