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Kinetic and binding parameters of BaSatAa
| Compound | Km (app) (μM) | Kcat (s−1) | Kcat/Km (M−1 s−1) | Kd (μM) |
|---|---|---|---|---|
| Streptothricin | 2.8 ± 0.4 | 2.5 × 103 | 8.9 × 108 | 3.7 |
| Acetyl-CoA | 32 ± 7 | 3.3 × 103 | 1.0 × 108 | ND |
Kinetic parameters were obtained from technical triplicates in two separate experiments. ND, not determined.
