TABLE 6.
Plasmids used in this study
| Plasmid | Allele/description | Encoded variant | Referencea |
|---|---|---|---|
| Derivatives of plasmid pCV1b | |||
| pBsSATA1 | B. subtilis satA+ | BsSatAWT | 26 |
| pBsSATA10 | B. subtilis satA2 | BsSatAL128F | |
| pBsSATA11 | B. subtilis satA3 | BsSatAL126F | |
| pBsSATA12 | B. subtilis satA4 | BsSatAA137T | |
| pBsSATA13 | B. subtilis satA5 | BsSatAS84C | |
| pBsSATA4 | B. subtilis satA6 | BsSatAE93Q | |
| pBsSATA9 | B. subtilis satA7 | BsSatAE129Q | |
| pBaSAT1 | B. anthracis satA+ | BaSatAWT | 26 |
| pBaSAT3 | B. anthracis satA1 | BaSatAY149A | |
| pBaSAT4 | B. anthracis satA2 | BaSatAY149F | |
| pBaSAT5 | B. anthracis satA3 | BaSatAF154A | |
| pBaSAT6 | B. anthracis satA4 | BaSatAF154W | |
| pBaSAT7 | B. anthracis satA5 | BaSatAD160E | |
| pBaSAT8 | B. anthracis satA6 | BaSatAD160G | |
| pBaSAT9 | B. anthracis satA7 | BaSatAY164A | |
| pBaSAT10 | B. anthracis satA8 | BaSatAY164F | |
| pBaSAT11 | B. anthracis satA9 | BaSatAY164W | |
| pBaSAT12 | B. anthracis satA10 | BaSatAY149L | |
| pBaSAT13 | B. anthracis satA11 | BaSatAY149W | |
| pBaSAT14 | B. anthracis satA12 | BaSatAF154G | |
| pBaSAT15 | B. anthracis satA13 | BaSatAF154L | |
| pBaSAT16 | B. anthracis satA14 | BaSatAF154Y | |
| pBaSAT17 | B. anthracis satA15 | BaSatAD160A | |
| pBaSAT18 | B. anthracis satA16 | BaSatAY164G | |
| pBaSAT19 | B. anthracis satA17 | BaSatAY164L | |
| pBaSAT20 | B. anthracis satA18 | BaSatAY149G | |
| pBaSAT21 | B. anthracis satA19 | BaSatAD160N | |
| pTEV5 | TEV protease-cleavable, N-terminal His6 tag overexpression vector | 45 | |
| Derivatives of plasmid pTEV5c | |||
| pBaSAT2 | B. anthracis satA+ | BaSatAWT | 26 |
| pBaSAT22 | B. anthracis satA1 | BaSatAY149A | |
| pBaSAT23 | B. anthracis satA2 | BaSatAY149F | |
| pBaSAT24 | B. anthracis satA18 | BaSatAY149G | |
| pBaSAT25 | B. anthracis satA10 | BaSatAY149L | |
| pBaSAT26 | B. anthracis satA11 | BaSatAY149W | |
| pBaSAT27 | B. anthracis satA3 | BaSatAF154A | |
| pBaSAT28 | B. anthracis satA12 | BaSatAF154G | |
| pBaSAT29 | B. anthracis satA13 | BaSatAF154L | |
| pBaSAT30 | B. anthracis satA4 | BaSatAF154W | |
| pBaSAT31 | B. anthracis satA14 | BaSatAF154Y | |
| pBaSAT32 | B. anthracis satA15 | BaSatAD160A | |
| pBaSAT33 | B. anthracis satA5 | BaSatAD160E | |
| pBaSAT34 | B. anthracis satA6 | BaSatAD160G | |
| pBaSAT35 | B. anthracis satA19 | BaSatAD160N | |
| pBaSAT36 | B. anthracis satA7 | BaSatAY164A | |
| pBaSAT37 | B. anthracis satA8 | BaSatAY164F | |
| pBaSAT38 | B. anthracis satA16 | BaSatAY164G | |
| pBaSAT39 | B. anthracis satA17 | BaSatAY164L | |
| pBaSAT40 | B. anthracis satA9 | BaSatAY164W | |
| pTEV16 | TEV protease-cleavable, N-terminal His6 tag overexpression vector | 27 | |
| Derivatives of plasmid pTEV16d | |||
| pBsSATA2 | B. subtilis satA+ | BsSatAWT | 26 |
| pBsSATA14 | B. subtilis satA2 | BsSatAL128F | |
| pBsSATA15 | B. subtilis satA3 | BsSatAL126F | |
| pBsSATA16 | B. subtilis satA4 | BsSatAA137T | |
| pBsSATA17 | B. subtilis satA5 | BsSatAS84C |