TABLE 7.
Primers used in this study
| Primer | Sequence |
|---|---|
| BaSatA Y149A Forward | GCGTGCAAGTTCGCCGAAAAATGCGG |
| BaSatA Y149A Reverse | CCGCATTTTTCGGCGAACTTGCACGC |
| BaSatA Y149F Forward | CGTGCAAGTTCTTCGAAAAATGCGG |
| BaSatA Y149F Reverse | CCGCATTTTTCGAAGAACTTGCACG |
| BaSatA Y149G Forward | GCGTGCAAGTTCGGCGAAAAATGCGG |
| BaSatA Y149G Reverse | CCGCATTTTTCGCCGAACTTGCACGC |
| BaSatA Y149L Forward | GCGTGCAAGTTCCTCGAAAAATGCGGC |
| BaSatA Y149L Reverse | GCCGCATTTTTCGAGGAACTTGCACGC |
| BaSatA Y149W Forward | GCGTGCAAGTTCTGGGAAAAATGCGGC |
| BaSatA Y149W Reverse | GCCGCATTTTTCCCAGAACTTGCACGC |
| BaSatA F154A Forward | GAAAAATGCGGCGCTGTGATCGGTGG |
| BaSatA F154A Reverse | CCACCGATCACAGCGCCGCATTTTTC |
| BaSatA F154G Forward | GAAAAATGCGGCGGTGTGATCGGTGG |
| BaSatA F154G Reverse | CCACCGATCACACCGCCGCATTTTTC |
| BaSatA F154L Forward | GAAAAATGCGGCCTTGTGATCGGTG |
| BaSatA F154L Reverse | CACCGATCACAAGGCCGCATTTTTC |
| BaSatA F154W Forward | GAAAAATGCGGCTGGGTGATCGGTGGC |
| BaSatA F154W Reverse | GCCACCGATCACCCAGCCGCATTTTTC |
| BaSatA F154Y Forward | CGAAAAATGCGGCTATGTGATCGGTGGC |
| BaSatA F154Y Reverse | GCCACCGATCACATAGCCGCATTTTTCG |
| BaSatA D160A Forward | GATCGGTGGCTTCGCATTTCTGGTTTATAAG |
| BaSatA D160A Reverse | CTTATAAACCAGAAATGCGAAGCCACCGATC |
| BaSatA D160E Forward | GATCGGTGGCTTCGAATTTCTGGTTTATAAG |
| BaSatA D160E Reverse | CTTATAAACCAGAAATTCGAAGCCACCGATC |
| BaSatA D160G Forward | GATCGGTGGCTTCGGATTTCTGGTTTATAAG |
| BaSatA D160G Reverse | CTTATAAACCAGAAATCCGAAGCCACCGATC |
| BaSatA D160N Forward | CTTTGTGATCGGTGGCTTCAACTTTCTGGTTTATAAGG |
| BaSatA D160N Reverse | CCTTATAAACCAGAAAGTTGAAGCCACCGATCACAAAG |
| BaSatA Y164A Forward | GACTTTCTGGTTGCTAAGGGCCTGAAC |
| BaSatA Y164A Reverse | GTTCAGGCCCTTAGCAACCAGAAAGTC |
| BaSatA Y164F Forward | GACTTTCTGGTTTTTAAGGGCCTGAAC |
| BaSatA Y164F Reverse | GTTCAGGCCCTTAAAAACCAGAAAGTC |
| BaSatA Y164G Forward | GACTTTCTGGTTGGTAAGGGCCTGAAC |
| BaSatA Y164G Reverse | GTTCAGGCCCTTACCAACCAGAAAGTC |
| BaSatA Y164L Forward | CGACTTTCTGGTTCTTAAGGGCCTGAAC |
| BaSatA Y164L Reverse | GTTCAGGCCCTTAAGAACCAGAAAGTCG |
| BaSatA Y164W Forward | GACTTTCTGGTTTGGAAGGGCCTGAAC |
| BaSatA Y164W Reverse | GTTCAGGCCCTTCCAAACCAGAAAGTC |
| BaSatA Y164S Forward | GACTTTCTGGTTTCCAAGGGCCTGAAC |
| BaSatA Y164S Reverse | GTTCAGGCCCTTGGAAACCAGAAAGTC |
| BaSatA Y149S Forward | CGTGCAAGTTCTCCGAAAAATGCGG |
| BaSatA Y149S Reverse | CCGCATTTTTCGGAGAACTTGCACG |
| BsSatA E93Q | GTATGCACTAATACAGGACATTGCCG |
| BsSatA E129Q Forward | CATTTTTGTGGTCTTATGCTTCAGACCCAAGATATTAATATTTC |
| BsSatA E129Q Reverse | GAAATATTAATATCTTGGGTCTGAAGCATAAGACCACAAAAATG |