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. 2019 Mar 6;85(6):e02586-18. doi: 10.1128/AEM.02586-18

TABLE 2.

Primers used in this studya

Primer name Sequence (5→3)
Fw_fliA_UP GTGAATTCGGCCAACTGGCGGAGATCTT
Rev_fliA_UP GGATCCCTTAGGTCGTCTCTACTTGCCTTTTTTTCCCTTGAC
Fw_fliA_DOWN TAGAGACGACCTAAGGGATCCCTGCGCGAGCATTCCTGGAGCG
Rev_fliA_DOWN GGTTAAGCTTGTCGAAAAACTGCGCCAGGA
Fw_phaR_UP GAGGATCCCGTGACCGTCAACACCGTCT
Rev_phaR_UP AGATCTCTTAGGTCGTCTCTATGCAGTAGTCATCTGAAGTCCAGTC
Fw_phaR_DOWN TAGAGACGACCTAAGAGATCTATGTTCGGCACCTTCCCC
Rev_phaR_DOWN GTTCTGCAGTTGCCGCGATTCATGGTGG
Fw_phaZ1_UP TCGGATCCCAAGCAACTGAAGGTGAT
Rev_phaZ1_UP GATATCCTTAGGTCGTCTCTACTTGGCTGAGGTCTCCGC
Fw_phaZ1_DOWN TAGAGACGACCTAAGGATATCTACGGCATCTTCTCGGGC
Rev_phaZ1_DOWN TCTCTAGATGCTGATGCAATCGACCG
Fw_phaZ2_UP TCGGATCCCCATACCAGTACGTCGCCA
Rev_phaZ2_UP AGATCTCTTAGGTCGTCTCTACAGCGCCTGCGCCATCATC
Fw_phaZ2_DOWN TAGAGACGACCTAAGAGATCTCATTACGGCGTCTTCAAC
Rev_phaZ2_DOWN TTCTGCAGTGAATTCGGTGGTCTTCT
Fw_phaRc_Hs TGTCTGCAGCGGTTCGGACTTCTCCCTCA
Rev_phaRc_Hs TGTGAGCTCTGCACTTCCGGAGCCTTTACCTGC
Fw_phaC1_prom GGGGACAAGTTTGTACAAAAAAGCAGGCTACTTTTCGGCGCTGCTTCTTC
Rev_phaC1_prom GGGGACCACTTTGTACAAGAAAGCTGGGTCCAGAACTTCGGATCGGACAT
Fw_phaZ1_prom GGGGACAAGTTTGTACAAAAAAGCAGGCTAAGGCAAGAACATCGTCATCT
Rev_phaZ1_prom GGGGACCACTTTGTACAAGAAAGCTGGGT TTCATGCAGTTGATAAAGCAT
Fw_phaZ2_prom GGGGACAAGTTTGTACAAAAAAGCAGGCTCGATGTCCTCCGGTTCCTTCT
Rev_phaZ2_prom GGGGACCACTTTGTACAAGAAAGCTGGGTGAGCTGATAGGTAGGGATCAT
a

The primers were generated in this study.