TABLE 3.
Primers used in this study
| Primer function and name | Sequence (5′–3′) |
|---|---|
| Deletion | |
| L-CgRDS2-F | ATTCGAAGGCCCACTGTA |
| L-CgRDS2-R | ACCCTCTTAACAAACGCCATGTCAAAAATATGATGCTGTGCTTAG |
| CgHIS3(CgRDS2)-F | CACAGCATCATATTTTTGACATGGCGTTTGTTAAGAGGGT |
| CgHIS3(CgRDS2)-R | ACTTGTCTATGCATATGTGTCTATGCTAGGACACCCTTAGT |
| R-CgRDS2-F | CTAAGGGTGTCCTAGCATAGACACATATGCATAGACAAGTTATATACA |
| R-CgRDS2-R | CCACTATTAGTGGCCCTAAATAAGT |
| L-CgHOG1-F | CAGAGGTCTTCATTCTGATCACTCTC |
| L-CgHOG1-R | AGTAACGAATCAAATGACATTATTTCTTTCTGGTGTTCCTATTTAATTAA |
| CgTRP1(CgHOG1)-F | CACCAGAAAGAAATAATGTCATTTGATTCGTTACTCGACA |
| CgTRP1(CgHOG1)-R | AATTAAATTTTATATGTAAGTCATTGTTTCTTTGCATTTTGTACAT |
| R-CgHOG1-F | GCAAAGAAACAATGACTTACATATAAAATTTAATTATTCGTCTTCCT |
| R-CgHOG1-R | TGCATTAATGAGTTGCAATTATTACTAA |
| Overexpression | |
| CgRDS2-F | AAGGAAAAAAGCGGCCGCATGGAAGAACCAGCAGC |
| CgRDS2-R | GGAAGATCTTTAGTTGGAATGATCTCTTGTAGGA |
| CgRDS22A-F | AAGGAAAAAAGCGGCCGCATGGAAGAACCAGCAGC |
| CgRDS22A-R | GGAAGATCTTTAGTTGGAATGATCTCTTGTAGGA |
| CgRDS22A-Myc-F | AAGGAAAAAAGCGGCCGCATGGAAGAACCAGCAGC |
| CgRDS22A-Myc-R | GGAAGATCTTTACAGATCCTCTTCAGAGATGAGTTTCTGCTCGTTGGAATGATCTCTTGTAG |
| Point mutation | |
| CgRds2/S64A-F | AATAGTATGGCGCCAATAGG |
| CgRds2/S64A-R | CCTATTGGCGCCATACTATT |
| CgRds2/T97A-F | GGTACAGAAGCACCAATATC |
| CgRds2/T97A-R | GATATTGGTGCTTCTGTACC |
| CgRds2/T238A-F | TATGACGATGCACCCACATC |
| CgRds2/T238A-R | GATGTGGGTGCATCGTCATA |
| CgRds2/S455A-F | ATCCCACTAGCTCCTACAAG |
| CgRds2/S455A-R | CTTGTAGGAGCTAGTGGGAT |
| Yeast two-hybrid | |
| BD-CgRDS2-F | GGGCCTCGAGCCCGGGTCGACATGGAAGAACCAGCAGCCAA |
| BD-CgRDS2-R | CTATAGGGCTCTAGAGTCGACTTAGTTGGAATGATCTCTTGTAGGAGA |
| AD-CgHOG1-F | AAAGAGATCGAATTAGGATCCATGGCTACTAATGAAGAGTTCATAAGAAC |
| AD-CgHOG1-R | TTCTCTGCTAGCAGAGGATCCTTATTGTTGGAATTCATTTGCAAAA |
| Coimmunoprecipitation | |
| pY26/PTEF-CgRDS2-F | AAGGAAAAAAGCGGCCGCATGGAAGAACCAGCAGC |
| pY26/PTEF-CgRDS2-R | GGAAGATCTTTACAGATCCTCTTCAGAGATGAGTTTCTGCTCGTTGGAATGATCTCTTGTAG |
| pY26/PGPD-CgHOG1-F | CAGGAATTCGATATCAAGCTTATGGCTACTAATGAAGAGTTCATAAGAAC |
| pY26/PGPD-CgHOG1-R | GTCGACGGTATCGATAAGCTTTTAAGCGTAGTCTGGGACGTCGTATGGGTATTGTTGGAATTCATTTGCAA |
| RT-PCR and ChIP | |
| CgLCA1-F | AGACCTGGCTATTATATGA |
| CgLCA1-R | CCGAATGACAACTTGATA |
| CgDPP1-F | GAGTTAATGATGTGATGTTATTC |
| CgDPP1-R | TCAGTCTTCCAATCCAAT |
| CgPGS1-F | CAAGTATAGCACCGAATC |
| CgPGS1-R | CAGTAGTTATCTTCTTCTTCA |
| CgGEP4-F | AGAGACGAGATTATTGAAT |
| CgGEP4-R | CTTACTGAAGGCACTATT |
| CgCHO1-F | GATTGTGAGTTGCCTGAG |
| CgCHO1-R | ACCTAAGAATATGAAGAAGTGT |
| CgPSD1-F | AAGATGAGGAGGCAACTG |
| CgPSD1-R | TTAGGTGAGACTTCGGTTAG |
| CgCPT1-F | CACAATAAGAAGAATGACA |
| CgCPT1-R | GTTAGATGACCAACAATC |
| CgCKI1-F | GAGCAGGAATCTACTAAGG |
| CgCKI1-R | TGTATCTATGTCATCTACCAAT |