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. Author manuscript; available in PMC: 2019 Oct 16.
Published in final edited form as: Anal Chem. 2018 Sep 28;90(20):11873–11882. doi: 10.1021/acs.analchem.8b01913

Figure 5.

Figure 5.

Relative abundance of markers representing exosomes and subcellular organelles (A) and drug metabolizing enzymes (B) in human hepatocyte Culture media collected at 24, 48 and 72 hours in sandwich culture (mean±S.E., n=4). Detected exosomal markers were: TGM2 and CD26. Different organelles were characterized by following proteins: Nuclear (Lamin B1), mitochondrial (Hsp60), cytoskeleton (Vimentin and Desmin), ER membrane (NADPH CYP450 reductase), ER lumen (Calreticulin), peroxisomes (Catalase), and cytosol (Hsp-90 beta and LDH-H). *P<0.05 and **P<0.01 indicates statistically significant variation which was analyzed by One-way ANOVA followed by Bonferroni post-test (24hours as control).