FIGURE 1.

Summary of the regulatory mechanisms of Ψ-lncs and the analysis pipeline for the identification of their deregulation in lung adenocarcinoma. (A) Summary of the regulatory mechanisms of pseudogene-derived lncRNAs (Ψ-lncs) that retain sequence homology with the parent gene. Overall, lncRNAs have been shown to function through a variety of regulatory mechanisms, acting on the DNA, RNA, and protein levels. (B) Flow diagram description of the analysis pipeline applied for the identification of deregulated Ψ-lncs. Patient LUAD samples were collected and subjected to next generation sequencing to quantify RNA expression. Gene expression was then compared between tumors and matched non-malignant tissue to identify significantly deregulated transcripts. LncRNAs with exonic overlap to known pseudogenes were then identified and confirmed in a 2nd set of LUAD and matched non-malignant tissue. Deregulated Ψ-lncs were then assayed to determine associations with clinical features.