Table 3.
Quantified host proteins involved in several metabolic processes (glycolysis, pentose phosphate pathway, TCA cycle, lipid metabolism, and oxidative phosphorylation) and their respective fold change in abundance upon infection of THP-1 macrophages with R. conorii (Rc/uninf) or R. montanensis (Rm/uninf).
| Pathway | Name | UniProt | EC number | Log2 (Rc/uninf) | Log2 (Rm/uninf) |
|---|---|---|---|---|---|
| Glycolysis | Phosphoglucomutase 2 (PGM2) | Q96G03 | 5.4.2.2 | −0.59 | −0.64 |
| Glucose-6-phosphate isomerase (GPI) | P06744 | 5.3.1.9 | −0.41 | −0.40 | |
| Fructose-1,6-biphosphatase I (FBP1) | P09467 | 3.1.3.11 | −0.04 | −0.33 | |
| Phosphofructokinase, liver type (PFKL) | P17858 | 2.7.1.11 | 0.09 | −0.15 | |
| Aldolase, fructose-biphosphate A (ALDOA) | P04075 | 4.1.2.13 | −0.39 | −0.30 | |
| Triosephosphate isomerase (TPI1) | P60174 | 5.3.1.11 | −0.48 | −0.40 | |
| Glyceraldehyde-3-phosphate dehydrogenase (GADPH) | P04406 | 1.2.1.12 | −0.56 | −0.42 | |
| Phosphoglycerate kinase 1 (PGK1) | P00558 | 2.7.2.3 | −0.60 | −0.62 | |
| Phosphoglycerate mutase 1 (PGAM1) | P18669 | 5.4.2.11 | −0.75 | −0.60 | |
| Enolase 1 (ENO1) | P06733 | 4.2.1.11 | −0.48 | −0.39 | |
| Pyruvate kinase, muscle (PKM) | P14618 | 2.7.1.40 | −0.45 | −0.47 | |
| Lactate dehydrogenase A(LDHA) | P00338 | 1.1.1.27 | −0.24 | −0.38 | |
| Lactate dehydrogenase B(LDHB) | P07195 | 1.1.1.27 | −0.27 | −0.38 | |
| Pentose Phosphate Pathway | Glucose-6-phosphate dehydrogenase (G6PD) | P11413 | 1.1.149/1.1.1.343 | −0.22 | −0.29 |
| 6-phosphogluconolactonase (PGLS) | O95336 | 3.1.1.31 | −0.80 | −0.52 | |
| Phosphogluconate dehydrogenase (PGD) | P52209 | 1.1.1.44/1.1.1.343 | −0.48 | −0.25 | |
| Glucose-6-phosphate isomerase (GPI) | P06744 | 5.3.1.9 | −0.41 | −0.40 | |
| Transketolase (TKT) | P29401 | 2.2.1.1 | −0.64 | −0.60 | |
| Transaldolase 1 (TALDO1) | P37837 | 2.2.1.2 | −0.57 | −0.43 | |
| Phosphofructokinase, liver type (PFKL) | P17858 | 2.7.1.11 | 0.09 | −0.15 | |
| Fructose-biphosphatase 1 (FBP1) | P09467 | 3.1.3.11 | −0.04 | −0.33 | |
| Aldolase, fructose-biphosphatase A (ALDOA) | P04075 | 4.1.2.13 | −0.39 | −0.30 | |
| Phosphoglucomutase 2 (PGM2) | Q96G03 | 5.4.2.7/5.4.2.2 | −0.59 | −0.64 | |
| TCA cycle | Pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1) | P08559 | 1.2.4.1 | 0.40 | 0.17 |
| ATP citrate lyase (ACLY) | P53396 | 2.3.3.8 | −0.23 | −0.37 | |
| Citrate synthase (CS) | O75390 | 2.3.3.1 | 0.49 | 0.14 | |
| Aconitase 2 (ACO2) | Q99798 | 4.2.1.3 | 0.36 | 0.02 | |
| Isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2) | P48735 | 1.1.1.42 | 0.23 | −0.04 | |
| Isocitrate dehydrogenase 3 (NAD(+)) apha (IDH3A) | P50213 | 1.1.1.41 | 0.42 | −0.17 | |
| Isocitrate dehydrogenase (NADP(+)) 1, cytosolic (IDH1) | O75874 | 1.1.1.42 | −0.55 | −0.80 | |
| Fumarate hydratase (FH) | P07954 | 4.2.1.2 | 0.39 | 0.11 | |
| Malate dehydrogenase 2 (MDH2) | P40926 | 1.1.1.37 | 0.46 | 0.01 | |
| Malate dehydrogenase 1 (MDH1) | P40925 | 1.1.1.37 | −0.67 | −0.62 | |
| Dihydrolipoamide S-acetyltransferase (DLAT) | P10515 | 2.3.1.12 | −0.03 | 0.17 | |
| Dihydrolipoamide dehydrogenase (DLD) | P09622 | 1.8.1.4 | 0.58 | −0.11 | |
| Dihydrolipoamide S-succinyltransferase (DLST) | P36957 | 2.3.1.61 | 0.44 | 0.11 | |
| Glutamate dehydrogenase 1 (GLUD1) | P00367 | 1.4.1.3 | 0.28 | −0.02 | |
| Glutamic-oxaloacetic transaminase 2 (GOT2) | P00505 | 2.6.1.1 | 0.48 | 0.12 | |
| Malic enzyme 2 (ME2) | P23368 | 1.1.1.38 | 0.45 | 0.00 | |
| Lipid metabolism | Hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit (HADHB) | P55084 | 2.3.1.16 | 0.32 | −0.13 |
| hydroxyacyl-CoA dehydrogenase (HADH) | Q16836 | 1.1.1.35 | 0.20 | 0.13 | |
| Hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha subunit (HADHA) | P40939 | 1.1.1.211/4.2.1.17 | 0.55 | 0.14 | |
| Palmitoyl-protein thioesterase 1 (PPT1) | P50897 | 3.1.2.22 | −1.34 | −0.87 | |
| Hydroxysteroid 17-beta dehydrogenase 12 (HSD17B12) | Q53GQ0 | 1.1.1.330 | 0.50 | 0.25 | |
| Carnitine palmitoyltransferase 2 (CPT2) | P23786 | 2.3.1.21 | 0.25 | −0.10 | |
| Acyl-CoA dehydrogenase, C-4 to C-12 straight chain (ACADM) | P11310 | 1.3.8.7 | 0.12 | −0.33 | |
| Acyl-CoA dehydrogenase, very long chain (ACADVL) | P49748 | 1.3.8.9 | 0.25 | 0.07 | |
| Fatty acid synthase (FASN) | P49327 | 2.3.1.41 | 0.14 | 0.08 | |
| Apolipoprotein A1 (APOA1) | P02647 | – | 1.24 | 1.35 | |
| Apolipoprotein B (APOB) | P04114 | – | 1.35 | 0.54 | |
| Electron chain reaction (complex III) | Cytochrome c1 (CYC1) | P08574 | 1.10.2.2 | 0.34 | 0.17 |
| Ubiquinol-cytochrome c reductase core protein I (UQCRC1) | P31930 | 1.10.2.2 | 0.55 | 0.23 | |
| Ubiquinol-cytochrome c reductase core protein II (UQCRC2) | P22695 | 1.10.2.2 | 0.38 | 0.15 | |
| ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 (UQCRFS11) | P47985 | 1.10.2.2 | −0.16 | −0.46 | |
| Electron chain reaction (complex IV) | Cytochrome c oxidase subunit 4I1 (COX4I1) | P13073 | 1.9.3.1 | 0.42 | 0.85 |
| Cytochrome c oxidase subunit II (COX2) | P00403 | 1.9.3.1 | 0.36 | 0.12 | |
| Electron chain reaction (complex V) | ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle (ATP5A1) | P25705 | 3.6.3.14 | 0.43 | −0.05 |
| ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B) | P06576 | 3.6.3.14 | 0.47 | 0.10 | |
| ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 (ATP5C1) | P36542 | 3.6.3.14 | 0.39 | −0.05 | |
| ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit (ATP5D) | P30049 | 3.6.3.14 | 0.20 | 0.12 | |
| ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1 (ATP5F1) | P24539 | 3.6.3.14 | 0.35 | 0.24 | |
| ATPase H+ transporting V1 subunit A (ATP6V1A) | P38606 | 3.6.3.14 | 0.08 | 0.06 | |
| ATPase H+ transporting V1 subunit E1 (ATP6V1E1) | P36543 | 3.6.3.14 | −0.33 | −0.36 | |
| ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit (ATP5O) | P48047 | 3.6.3.14 | 0.09 | 0.31 | |
| ATPase H+ transporting V0 subunit d1 (ATP6V0D1) | P61421 | 3.6.3.14 | 0.15 | −0.11 | |
| ATP synthase, H+ transporting, mitochondrial Fo complex subunit F2 (ATP5J2) | P56134 | 3.6.3.14 | 0.10 | −0.11 |
Proteins that are considered as altered (fold change ≤ 0.83 or fold change ≥ 1.2) between infected and uninfected conditions were color-coded according the following: decreased (blue), not changed (transparent), or increased (orange).