FIGURE 1.

Effects of scopoletin on intracellular free calcium [Ca²⁺]i levels in insect Sf9 cells. 0, 10, 50, and 90 μM indicate Sf9 cells incubated with scopoletin at concentrations of 0, 10, 50, and 90 μM, respectively for 24 h. (A) The [Ca²⁺]i level was detected by Fura-2/AM fluorescence staining. Positively stained calcium is shown as green zones in the captured images under a microscope. (B) The bar chart indicates the mean fluorescence intensity (MFI) of Fura-2/AM in Sf9 cells. Error bars represent the standard error of the calculated mean based on three biological replicates. Different letters on the error bars indicate significant difference according to Duncan’s multiple tests (alpha = 0.05). i.e., No statistical difference between “a” and “a”; significant difference among “a,” “b,” “c,” and “d.”