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RT-qPCR validation of some differentially expressed genes in RNA-seq. The relative expression levels of fifteen differentially expressed genes in scopoletin- or solvent-treated T. cinnabarinus were determined by RT-qPCR. RPS18 was used as the reference gene to normalize the gene expression using the ΔΔCq method. The y-axis indicates the log2 fold change of each gene in RT-qPCR and RNA-seq.
